source: AFRICAN JOURNAL OF BIOTECHNOLOGY
Abstract
Lysozyme from saliva of Bali cattle (Bos sondaicus) was successfully isolated through an aqueous mixture system of polyethylene glycol (PEG 4000) and salt (Na2SO4). About 100 µg/ml lysozyme of 14.2 kDa, determined through sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), per 4800 µg/ml saliva was obtained. Based on turbidity assay of Staphylococcus aureus, it was revealed that inhibition activities of 40 µg/ml isolated lysozyme were comparable to 800 µg/ml of a commercial hen egg white lysozyme. Key words: Lysozyme, Bali cattle, Bos sondaicus, PEG, aqueous mixture system.
Concepts :
Aquaculture disease management and microbiota
Glycosylation and Glycoproteins Research
Biochemical and Structural Characterization
article
cite 5
Year 2012
source AFRICAN JOURNAL OF BIOTECHNOLOGY
Citations by Year
| Year | Count |
|---|---|
| 2012 | 5 |