ANALISIS KERAGAMAN GENETIK MUTAN JARAK PAGAR (Jathropa curcas L.) HASIL PERLAKUAN MUTAGEN KOLKISIN BERDASARKAN PENANDA MOLEKULER RAPD

Authors : Herdiyana Fitriani
article cite 0 Year 2013
source: Bioscientist : Jurnal Ilmiah Biologi
Abstract

The purpose of this research is to analyze genetic diversity of ( Jathropa curcas L. ), including genotypic expression through observation of DNA fragment tape by RAPD technique equipped with phenotype data. Samples in this research were 19 mutant ( Jatrohpa curcas L. ) ie mutant plants 0.1% + 6x, 0.2% + 6x, 0.3% + 6x, 0.4% + 6x, 0.5 % + 6x, 0.6% + 6x, 0.1% + 8x, 0.2% + 8x, 0.3% + 8x, 0.4% + 8x, 0.5% + 8x, 0.6% + 8x, 0.1% + 10x, 0.2% + 10x, 0.3% + 10x, 0.4% + 10x, 0.5% + 10x, and 0.6% + 10x and controls. The type of research is descriptive explorative research. Phenotypic observation results, indicating that there are many differences in ( Jathropa curcas L. ) and control. This difference is seen from the number of leaves, shoot color, stem color, number of main branches, plant height, and stem diameter. The differences that occur in morphological characters are caused by environmental influences. Genotypic observations in the form of DNA bands showed that the total number of amplified DNA bands using primary OPA 20, OPH 20 and OPH 15 were 169 DNA bands. Based on the results of the analysis using dendogram showed that the diversity of 19 ( Jathropa curcas L. ) plants is very high, with similarity level is at 52% coefficient. While mutants that have the closest kinship to the control are 0.6% + 10x mutants with similarity levels are at 84% coefficient.


Concepts :
Plant Genetic and Mutation Studies
GABA and Rice Research
Medicinal Plant Research
article cite 0 Year 2013 source Bioscientist : Jurnal Ilmiah Biologi
Access to Document
10.33394/bjib.v1i1.776
SDGs
Life in Land
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