Abstract

The study aimed to evaluate the viability of probiotics bacteria used in the production of synbiotics. This study used factorial completely randomized design 4 × 2 with 3 replications. The first treatment consisted of four bacteria isolates including B 299 isolates, C 610 isolates, D 610 isolates, and E 610 isolates. The second treatment was two prebiotics including rice bran prebiotics supplemented by egg hatching waste and rice bran prebiotics without supplemented. The suspension of probiotics bacteria (OD 600 : 0,2) was sprayed 6 ml into 20 g prebiotics relatively and dried in an incubator with temperature 43 oC for 24 hours. The measure of bacteria viability was carried out using the spread plate method on the first, third and fifth days. The results of this study showed that the strains of probiotics bacteria and prebiotics and interactions both did not show a significant difference (P>0,05) to the viability of probiotics bacteria; the average number of probiotics bacteria in rice bran prebiotics supplemented by egg hatching waste was 5,68 ± 1,14 log CFU/g for B 299 isolates, 4,76 ± 1,43 log CFU/g for C 610 isolates, 5,84 ± 1,49 log CFU/g for D 610 isolates and 6,98 ± 1,39 log CFU/g for E 610 isolates; the average number of probiotics bacteria in rice bran prebiotics without supplemented was 5,76 ± 1,61 log CFU/g for B 299 isolates, 6,65 ± 1,24 log CFU/g for C 610 isolates, 5,84 ± 1,31 log CFU/g for D 610 isolates and 7,10 ± 0,41 log CFU/g for E 610 isolates. In summary, the results might determine that the probiotics bacteria have grown with the same viability in synbiotics.

Concepts :

Access to Document

SDGs

Citations by Year