Abstract
This research studies the efficient method for heavy metal analysis in marine fish samples by atomic absorption spectrophotometry. The dissolution of the samples used the wet destruction technique in 2 ways. The first method uses a mixture of HNO3, H2SO4, HClO4 (1:2:1), and the second method uses a mixture of HNO3and H2SO4 (1:1). Tuna (Euthynnus affinis) was taken as Marine fish samples. The results showed that the first method took 180 minutes with a % recovery of 98.79% - 99.55%. In contrast, the second method took 240 minutes and a % recovery of 98.08% - 98.45%. The results of measurements by atomic absorption spectrophotometry (AAS) using standard calibration curve and standard addition techniques are as follows: using standard regression curve technique obtained Pb (0.664 ± 0.067) mg/kg, Cu (2.780 ± 0.01) mg/kg, Cd (0.192 ± 0.044) mg/kg, and Zn (1.824 ± 0.075) mg/kg, while the standard addition method obtained Pb (0.612 ± 0.016) mg/kg, Cu (2.364 ± 0.016) mg/kg, Cd (0.148 ± 0.029) mg/kg and Zn (1.692 ± 0.016) mg/kg. The calculation of the level of accuracy of the analysis showed that the standard addition method RSD gave a precision value of 0.97% - 3.76% and the standard curve method RSD with a precision of 5.76% - 31.77%. In conclusion, (1) Heavy metal content in tuna (Euthynnus affinis) is still within the permissible limits. (2) wet digestion technique using a mixture of HNO3, H2SO4, HClO4 (1:2:1) can be more efficient. (3) The measurement results of the standard addition method give more precise results