Abstract

The use of probiotics is increasingly popular as an alternative replacement to antibiotic uses in stimulating health and growth of broiler chickens. The use of antibiotics may disturb the balance of the gut microbiota and play a significant role in the emerging of antibiotic resistance pathogens. Thus, the objective of this study was to isolate and screen lactic acid bacteria (LAB) from the intestine of broiler chickens as probiotic candidates. LAB were initially screened by phenotypic assay. Furthermore, those strains which belonged to LAB were screened for inhibitory activity against E. coli. Results showed that a total of 4 isolates were considered as members of lactic acid bacteria indicating by the results of phenotypic assays: gram positive, catalase and oxidase negative, and able to perform carbohydrate fermentation. Out of the 4 isolates, only one isolate showed an inhibitory activity against the targeted pathogen. Further research is still needed to confirm the potential strain for probiotic in poultry.

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