Abstract

Dysregulation of the oncoprotein c-Myc (Myc) is involved in many types of cancer. Myc is a sequence-specific transcription factor that regulates the transcription of genes involved in the control of cell proliferation and apoptosis through mechanisms that are not well understood. The method of this research is experimental. The experiment result will be described. Some processes will be done by polymerase chain reaction (PCR) to amplify the gene and then it will be extracted to pure the targeted gene. This research has been succesful to purify 3×Myc PKG-Puro-Poly A gene. c-MYC (hereinafter MYC) is an oncoprotein consisting of 439 amino acids contains a well-characterized C-terminal DNA compound and an N-terminal transactivation domain (TAD). The C-terminal region »100 residues comprises a basic leucine zipper-helix-loop-helix (bHLH-LZ) segment that regulates heterodimerization between MYC and its partner bHLH-LZ MAX mediate in their binding to gene promoters.

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