Abstract
Background: Wound healing is a complex process involving inflammation, proliferation, and tissue remodeling. Aloe vera and Vitamin E (Evion®) are well-known for their wound-healing properties because of their anti-inflammatory, antioxidant, and collagen-promoting effects. Aim: This study aimed to evaluate the comparative efficacy of Aloe vera leaf extract, Vitamin E, and their combined application on wound healing in rabbits (Oryctolagus cuniculus). Methods: Twenty male rabbits were divided into four groups: control (treated with saline), Aloe Vera, Vitamin E, and Combined (Aloe Vera + Vitamin E). Full-thickness wounds were created on the dorsum of each rabbit, and treatments were applied daily for 14 days. Wound closure rates were measured on days 0, 4, 7, 10, and 14 followed by histopathological and biochemical analyses. Results: By day 14, the combined treatment group demonstrated the most significant wound closure, reducing the wound area by 75% compared with 50% in the Aloe Vera group, 55% in the Vitamin E group, and 35% in the control group. Histopathological analysis showed near-complete re-epithelialization, dense granulation tissue, and enhanced collagen deposition in the combined treatment group. The biochemical results indicated that the hydroxyproline levels were highest in the combined group (6.0 mg/g tissue) and that the antioxidant enzyme activity was elevated. Conclusion: These findings suggest a synergistic effect between Aloe Vera and Vitamin E, leading to accelerated wound healing and improved tissue repair. The combination treatment has the potential to be a superior therapeutic approach for wound management.