Abstract

Semen contains various biochemical substances, both beneficial for life functions and toxic substances that can damage spermatozoa, such as metabolic wastes and free radicals. Semen washing will optimize spermatozoa fertility, as harmful substances and the main energy source are removed. Therefore, giving fructose or glucose is necessary for energy recovery. The washed Boer Bucks semen was stored in Tris-egg yolk (TEY) without glucose or fructose (T1), TEY plus 1% glucose (TEYG, T2), and TEY plus 1% fructose (TEYF, T3). Each treatment was repeated 5 times to observe motility, viability, abnormalities, and intact plasma membrane (IPM) spermatozoa. We analyzed the data using analysis of variance (ANOVA) and the SPSS-20 application. In 8 hours of storage, the results indicated that the average motility of spermatozoa in T1, T2, and T3 was 42.00 ± 1.67%, 44.33 ± 2.88%, and 49.00 ± 2.53%. The viability percentages were 45.67 ± 1.21%, 48.00 ± 1.41%, and 53.50 ± 2.59%, while the abnormality percentages were 15.33 ± 0.52%, 14.50 ± 1.05%, and 12.17 ± 1.17%. The IPM was 42.67 ± 1.21%, 44.50 ± 1.05%, and 49.33 ± 1.75%. In conclusion, glucose or fructose can maintain the quality of spermatozoa post-washed, with the best quality obtained from 1% fructose/100 ml of diluent. Contribution to Sustainable Development Goals (SDGs):SDG 2: Zero HungerSDG 12: Responsible Consumption and Production

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