High-Performance Urease-BTB/Silicalite Optical Biosensor for Real-Time Urea Detection

Authors : Dhony Hermanto; Rochmad Krissanjaya; Nurul Ismillayli; Lisa Savitri; Rasyadan Taufiq Probojati
preprint cite 0 Year 2025
source: ChemRxiv
Abstract

A urease–BTB/silicalite optical biosensor was developed for real-time urea detection. The silicalite matrix provided a stable platform for urease immobilization, enabling reliable enzymatic activity and a measurable reflectance response. The biosensor demonstrated high sensitivity, reproducibility, and selectivity, with strong agreement to standard methods. Successful detection in environmental water samples highlights its applicability for urea monitoring in complex matrices.


Concepts :
Advanced biosensing and bioanalysis techniques
Electrochemical sensors and biosensors
Analytical Chemistry and Sensors
preprint cite 0 Year 2025 source ChemRxiv
SDGs
Clean water and sanitation
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